Frederick Tan
Frederick Tan
Bioinformatics
Lab Contacts:
Office (410) 246-3084
Lab (410) 246-3083
Fax (410) 243-6311
Lab Members: 
Katherine Cox, Visiting Post-Doc
Michael Diamreyan, Research Assistant

RESEARCH SUMMARY

My research in yeast focuses on the fundamental question: What factors make a given molecule a “good substrate” for homologous recombination?  Such factors determine how often non-allelic repetitive DNA recombines to produce chromosomal aberrations associated with cancer and hereditary diseases.  To address this question, I am developing genome-wide tools to determine what factors are most important in governing recombination between naturally occurring repeats.  In addition to spontaneous recombination events, recombination machinery can also be harnessed as a powerful tool to precisely modify genomes.  I am using genetic screens to identify rate-limiting steps during genome modification.

PUBLICATIONS

  • Brawand D, et al. (2014).  The genomic substrate for adaptive radiation in African cichlid fish.  Nature(pmid 25186727)

  • * Tan FJ, * Hoang ML, and Koshland D (2012).  DNA resection at chromosome breaks promotes genome stability by constraining non-allelic homologous recombination.  PLoS Genetics (pmid 22479212)

  • Hoang ML, Tan FJ, Lai DC, Celniker SE, Hoskins RA, Dunham MJ, Zheng Y, and Koshland D (2010).  Competitive repair by naturally dispersed repetitive DNA during non-allelic homologous recombination.  PLoS Genetics (pmid 21151956)

  • * Tan FJ, * Zuckerman JE, Wells RC, and Hill RB (2011).  The C. elegans B-cell lymphoma 2 (Bcl-2) homolog cell death abnormal 9 (CED-9) associates with and remodels lipid membranes.  Protein Science(pmid 21031486)

  • Tan FJ, Husain M, Manlandro CM, Koppenol M, Fire AZ, and Hill RB (2008).  CED-9 and mitochondrial homeostasis in C. elegans muscle.  J Cell Science (pmid 18827010)

  • Tan FJ, Fire AZ, and Hill RB (2007).  Regulation of apoptosis by C. elegans CED-9 in the absence of the C-terminal transmembrane domain.  Cell Death & Differentiation (pmid 17703231)

  • Wells RC, Picton LK, Williams SC, Tan FJ, and Hill RB (2007).  Direct binding of the dynamin-like GTPase, Dnm1, to mitochondrial dynamics protein Fis1 is negatively regulated by the Fis1 N-terminal arm.  J Biological Chemistry (pmid 17884824)

  • * Johnson SM, * Tan FJ, McCullough HL, Riordan DP, and Fire AZ (2006).  Flexibility and constraint in the nucleosome core landscape of Caenorhabditis elegans chromatin.  Genome Research (pmid 17038564)

  • Fire A, Alcazar R, and Tan F (2006).  Unusual DNA structures associated with germline genetic activity in Caenorhabditis elegans.  Genetics (pmid 16648589)

Co-first authors