Allan Spradling
Allan Spradling
Staff Member
Lab Contacts:
Office (410) 246-3015
Lab (410) 246-3038
Fax (410) 243-6311

Postdoctoral Associates

Steven DeLuca    (HHMI)
Kun Dou    
Ethan Greenblatt    (HHMI)
Wanbao Niu    (HHMI)
John Urban    (HHMI)
Chenhui Wang    (HHMI)

Graduate Students

Haolong Zhu    

Research Scientists

Technicians

Margaret Collins    (HHMI)
Carol Davenport    (HHMI)
Dianne Williams    (HHMI)

  Oogenesis is the start of every animal's life.  This basic, conserved program is essential for metazoan development and also provides unparalleled opportunities to understand stem cells, the cell cycle, metabolism, chromatin structure, gene regulation, protein synthesis, transposon control, patterning, intercellular signaling and morphogenesis.  By studying both Drosophila and mouse oogenesis, we have advanced our knowledge of the largely conserved genes and processes that are used to build and ovulate functional oocytes.  These include early germ cell development within interconnected cysts that supports transfer of nurse cell cytoplasm and organelles into oocytes, to form the Balbiani body.  Oocyte growth and ovulation in both systems are controlled by steroid hormones, insulin, neuropeptides and prostaglandins.  Metabolism is re-programmed to elicit nutrient storage, and to prepare the oocyte for a period of quiescence.  Ovulation is carried out by local proteolysis and leads to formation of a corpus luteum.  During all these steps, somatic ovarian cells play leading roles that have been greatly illuminated by genomic methods, revealing more evolutionary conservation.  Recent studies of stored oocytes provide insight into the importance of maintaining protein production to prevent meiotic instability, and compromised developmental capacity.  The basic knowledge revealed by these studies has potential applications for ameliorating infertility, improving in vitro fertilization, and preventing and/or treating birth defects, including autism spectrum disorders.  

 

A mouse ovarian germ cell cyst at embryonic day 14, showing intercellular bridges (green) connecting 21 individual germ cells (red). see Lei and Spradling,, 2016.